Sm. (TFDF) on osteoblasts (OB)
differentiation and the expression of p38 MAPK and BMP-2/Smads signaling in osteoblasts after treatment by Advanced Glycation End Products (AGEs), so as to explore the action mechanism for preventing and curing osteoporosis of TFDF. [Method] Primary osteoblasts of newborn Sprague-daweley rats were extracted and cultured by the double enzyme digestion method, and its biological characteristics were observed. pNPP, ELISA and Alizarin dyeing were respectively used to test ALP, Type I collagen (Col I), osteocalcin (BGP), BMP-2, and mineralization of osteoblasts after treatment by different concentration of AGEs and after treatment by different concentration of TFDF and AGEs (400 mg/L). Western-blot was
used to detect p38 and 还有 Smad1/5/8 protein phosphorylation after treatment by TFDF and AGEs (400 mg/L). [Result] The levels of ALP, Type I collagen, osteocalcin and mineralization in osteoblasts treated by different concentrations of AGEs (200, 400 and 800 mg/L) were lower than those in the control group. TFDF could increase ALP, Type I collagen, osteocalcin, BMP-2 and mineralization of osteoblasts in a dose-dependent manner after treatment by AGEs (400 mg/L). TFDF (50 mg/L) can increase protein phosphorylation of p38 and Smad1/5/8 in osteoblasts after treatment by AGEs (400 selleck chemical mg/L). [Conclusion] AGEs can decrease 什么 differentiation and mineralization of osteoblasts. TFDF can increase differentiation and mineralization of osteoblasts in a dose-dependent manner after treatment by AGEs, which may be related to p38 and BMP-2/Smads signaling.
本试验旨在研究丝裂原活化蛋白激酶(MAPK)信号通路抑制剂对断奶仔猪小肠形态和肠通透性的影响。选用体重约为5.8
kg的24头21日龄杜×长×大断奶仔猪,随机分成4组,每组6头。试验组断奶前30 min分别腹腔注射p38 MAPK抑制剂(SB203580,Ⅰ组)、c-Jun N末端激酶(JNK)抑制剂(SP600125,Ⅱ组)和胞外信号调节激酶1/2(ERK1/2)抑制剂(PD98059,Ⅲ组),对照组注射等量的生理盐水。于断奶后36 h屠宰仔猪取样待测。结果表明:Ⅰ组空肠绒毛高度和绒毛高度/隐窝深度均显著高于对照组(P<0.05),隐窝深度显著低于对照组(P<0.05);与对照组相比,Ⅰ组和Ⅱ组仔猪血浆D-乳酸、二胺氧化酶含量显著降低(P<0.05),而Ⅲ组仔猪血浆D-乳酸含量显著高于对照组(P<0.05);Ⅰ组空肠黏膜促炎细胞因子肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、白细胞介素6(IL-6)和干扰素γ(IFN-γ)水平显著低于对照组(P<0.05),与对照组相比,Ⅱ组TNF-α和IL-1β水平显著降低(P<0.05),而Ⅲ组TNF-α水平显著高于对照组(P0.05)。结果提示,在断奶应激致仔猪小肠黏膜屏障受损过程中,抑制p38 MAPK和JNK通路后,肠屏障得到改善,而抑制ERK1/2通路后肠屏障损伤有加重的趋势。
白细胞介素简称白介素,是指在白细胞或免疫细胞间相互作用的细胞因子,它在传递信息,激活与调节免疫细胞,介导T、B细胞活化、增殖与分化及在炎症反应中起重要作用。近期研究发现[1],IL-23/IL-17轴在炎症性疾病的发病机制中可能处于关键地位,并有望成为新的治疗靶标。IL-23
目的探讨我国汉族人群p38βMAPK基因多态性对mRNA表达的影响。方法纳入正常人群98名,经测序分析rs2072878位点AA基因型82名,AG基因型16名。分别选取AA与AG基因型组各14名,提取外周血单核细胞(PBMC),提取RNA与蛋白。p38βMAPK mRNA的测定采用实时荧光定量PCR,p38 MAPK总蛋白表达的检测采用SDS-PAGE电泳-蛋白免疫印迹法。结果 p38βMAPK rs2072878的AA基因型人群mRNA表达水平显著低于AG基因型人群[(0.485±1.143)vs(2.250±2.719),P=0.034]。p38MAPK蛋白表达水平AA基因型组与AG基因型组差异无统计学意义[(1.347±0.309)vs(1.007±0.500),P=0.